VPS33A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
VPS33A transfected lysate.
Western Blot
Lane 1: VPS33A transfected lysate ( 65.67 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VPS33A full-length
Host
Human
Theoretical MW (kDa)
65.67
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VPS33A antibody (H00065082-B01) by Western Blots.
SDS-PAGE Gel
VPS33A transfected lysate.
Western Blot
Lane 1: VPS33A transfected lysate ( 65.67 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VPS33A
Entrez GeneID
65082GeneBank Accession#
NM_022916.4Protein Accession#
NP_075067.2Gene Name
VPS33A
Gene Alias
FLJ22395, FLJ23187
Gene Description
vacuolar protein sorting 33 homolog A (S. cerevisiae)
Omim ID
610034Gene Ontology
HyperlinkGene Summary
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq
Other Designations
vacuolar protein sorting 33A
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