ACD monoclonal antibody (M02), clone 1D8-1B6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ACD on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 2 ug/ml]
Immunoprecipitation
Immunoprecipitation of ACD transfected lysate using anti-ACD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACD MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACD is 0.3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ACD on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (85.58 KDa) .
-
Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ACD.
Immunogen
ACD (AAH16904, 1 a.a. ~ 544 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTLIASPPSSPSSSGTPALPGHMSSEESGTSISLLPALSLAAPDPGQRSSSQPSPAICSAPATLTPRSPHASRTPSSPLQSCTPSLSPRSHVPSPHQALVTRPQKPSLEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEPPKRHRDGSAFQYEYEPPCTSLCARVQAARLPPQLMAWALHFLMDAQPGSEPTPM
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (59); Rat (61)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (85.58 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ACD on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 2 ug/ml]Immunoprecipitation
Immunoprecipitation of ACD transfected lysate using anti-ACD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACD MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACD is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ACD on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ACD
Entrez GeneID
65057GeneBank Accession#
BC016904Protein Accession#
AAH16904Gene Name
ACD
Gene Alias
PIP1, PTOP, TINT1, TPP1
Gene Description
adrenocortical dysplasia homolog (mouse)
Omim ID
609377Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I. [provided by RefSeq
Other Designations
POT1 and TIN2 organizing protein|TIN2 interacting protein 1|adrenocortical dysplasia homolog
-
Interactome
-
Disease
-
Publication Reference
-
An N-terminal Flag-tag impairs TPP1 regulation of telomerase function.
Sandhu R, Wei D, Sharma M, Xu L.
Biochemical and Biophysical Research Communications 2019 Apr; 512(2):230.
Application：IP, WB-Tr, Human, HEK 293T, HeLa cells.
-
Human Cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion.
Zanini IM, Soneson C, Lorenzi LE, Azzalin CM.
Journal of Cell Science 2017 Feb; 130(4):767.
Application：WB, Human , HeLa, U2OS cells.
-
The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres.
Frank AK, Tran DC, Qu RW, Stohr BA, Segal DJ, Xu L.
PLoS Genetics 2015 Jul; 11(7):e1005410.
Application：IF, WB, Human, HCT116 cells.
-
TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo.
Abreu E, Aritonovska E, Reichenbach P, Cristofari G, Culp B, Terns RM, Lingner J, Terns MP.
Molecular and Cellular Biology 2010 Jun; 30(12):2971.
Application：ChIP, IP, WB-Tr, Human, HeLa cells.
-
An N-terminal Flag-tag impairs TPP1 regulation of telomerase function.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com