MRPL1 monoclonal antibody (M02), clone 2C4
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More Files
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Western Blot (Cell lysate)
MRPL1 monoclonal antibody (M02), clone 2C4. Western Blot analysis of MRPL1 expression in A-431(Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of MRPL1 expression in transfected 293T cell line by MRPL1 monoclonal antibody (M02), clone 2C4.
Lane 1: MRPL1 transfected lysate(34.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MRPL1 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MRPL1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (59.07 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MRPL1.
Immunogen
MRPL1 (AAH15109, 1 a.a. ~ 303 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVYQTSLCSCSVNIRVPNRHFAAAKKSAKKTKKGAKEKTPDEKKDEIEKIKAYPYMEGEPEDDVYLKRLYPRQIYEVEKAVHLLKKFQILDFTSPKQSVYLDLTLDMALGKKKNVEPFTSVLSLPYPFASEINKVAVFTENASEVKIAEENGAASAGGTSLIQKIWDDEIVADFYVAVPEIMPELNRLRKKLNKKYPKLSRNSIGRDIPKMLELFKNGHEIKVDEERENFLQTKIATLDMSSDQIAANLQAVINEVCRHRPLNLGPFVVRAFLRSSTSEGLLLKIDPLLPKEVKNEESEKEDA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (78)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (59.07 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MRPL1 monoclonal antibody (M02), clone 2C4. Western Blot analysis of MRPL1 expression in A-431(Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of MRPL1 expression in transfected 293T cell line by MRPL1 monoclonal antibody (M02), clone 2C4.
Lane 1: MRPL1 transfected lysate(34.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MRPL1 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MRPL1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MRPL1
Entrez GeneID
65008GeneBank Accession#
BC015109Protein Accession#
AAH15109Gene Name
MRPL1
Gene Alias
BM022, FLJ96680, L1MT, MRP-L1
Gene Description
mitochondrial ribosomal protein L1
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family. [provided by RefSeq
Other Designations
39S ribosomal protein L1, mitochondrial|OTTHUMP00000160703
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Interactome
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Disease
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