MRPL40 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPL40 transfected lysate
Western Blot
Lane 1: MRPL40 transfected lysate ( 22.77 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL40 full-length
Host
Human
Theoretical MW (kDa)
22.77
Interspecies Antigen Sequence
Mouse (75); Rat (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL40 antibody (H00064976-B01) by Western Blots.
SDS-PAGE Gel
MRPL40 transfected lysate
Western Blot
Lane 1: MRPL40 transfected lysate ( 22.77 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL40
Entrez GeneID
64976GeneBank Accession#
BC009707Protein Accession#
AAH09707Gene Name
MRPL40
Gene Alias
FLJ41774, MGC9400, MRP-L22, NLVCF, URIM
Gene Description
mitochondrial ribosomal protein L40
Omim ID
605089Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Deletions in this gene may contribute to the etiology of velo-cardio-facial syndrome and DiGeorge syndrome. [provided by RefSeq
Other Designations
nuclear localization signal containing protein deleted in velocardiofacial syndrome|nuclear localization signal deleted in velocardiofacial syndrome|up-regulated in metastasis
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