MRPS11 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPS11 transfected lysate.
Western Blot
Lane 1: MRPS11 transfected lysate ( 21.45 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPS11 full-length
Host
Human
Theoretical MW (kDa)
21.45
Interspecies Antigen Sequence
Mouse (71); Rat (74)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPS11 antibody (H00064963-B02) by Western Blots.
SDS-PAGE Gel
MRPS11 transfected lysate.
Western Blot
Lane 1: MRPS11 transfected lysate ( 21.45 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPS11
Entrez GeneID
64963GeneBank Accession#
NM_022839.2Protein Accession#
NP_073750.2Gene Name
MRPS11
Gene Alias
FLJ22512, FLJ23406, HCC-2
Gene Description
mitochondrial ribosomal protein S11
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that contains a high level of sequence similarity with ribosomal protein S11P family members. A pseudogene corresponding to this gene is found on chromosome 20. Sequence analysis identified two transcript variants that encode different protein isoforms. [provided by RefSeq
Other Designations
cervical cancer proto-oncogene 2
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