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CENPH 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00064946-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CENPH full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 27.28
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CENPH antibody (H00064946-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00064946-T01
    CENPH transfected lysate
    Western Blot
    QC Testing of H00064946-T01
    Lane 1: CENPH transfected lysate ( 27.28 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • Gene Alias:
  • NNF1,PMF1
  • Gene Description:
  • centromere protein H
  • Gene Summary:
  • Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq
  • Other Designations:
  • NNF1, MIND kinetochore complex component, homolog,kinetochore protein CENP-H
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