CENPH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CENPH transfected lysate
Western Blot
Lane 1: CENPH transfected lysate ( 27.28 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CENPH full-length
Host
Human
Theoretical MW (kDa)
27.28
Interspecies Antigen Sequence
Mouse (65)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CENPH antibody (H00064946-B01) by Western Blots.
SDS-PAGE Gel
CENPH transfected lysate
Western Blot
Lane 1: CENPH transfected lysate ( 27.28 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CENPH
Entrez GeneID
64946GeneBank Accession#
BC012024Protein Accession#
AAH12024Gene Name
CENPH
Gene Alias
NNF1, PMF1
Gene Description
centromere protein H
Omim ID
605607Gene Ontology
HyperlinkGene Summary
Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq
Other Designations
NNF1, MIND kinetochore complex component, homolog|kinetochore protein CENP-H
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