CENPH (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of CENPH transfected lysate using rabbit polyclonal anti-CENPH and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-CENPH.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (65)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of CENPH transfected lysate using rabbit polyclonal anti-CENPH and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-CENPH.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-CENPH (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-CENPH (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — CENPH
Entrez GeneID
64946Gene Name
CENPH
Gene Alias
NNF1, PMF1
Gene Description
centromere protein H
Omim ID
605607Gene Ontology
HyperlinkGene Summary
Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq
Other Designations
NNF1, MIND kinetochore complex component, homolog|kinetochore protein CENP-H
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