NMNAT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NMNAT1 transfected lysate.
Western Blot
Lane 1: NMNAT1 transfected lysate ( 30.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NMNAT1 full-length
Host
Human
Theoretical MW (kDa)
30.8
Interspecies Antigen Sequence
Mouse (82); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NMNAT1 antibody (H00064802-B01) by Western Blots.
SDS-PAGE Gel
NMNAT1 transfected lysate.
Western Blot
Lane 1: NMNAT1 transfected lysate ( 30.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NMNAT1
Entrez GeneID
64802GeneBank Accession#
NM_022787.3Protein Accession#
NP_073624.2Gene Name
NMNAT1
Gene Alias
NMNAT, PNAT-1, PNAT1
Gene Description
nicotinamide nucleotide adenylyltransferase 1
Omim ID
608700Gene Ontology
HyperlinkGene Summary
The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM
Other Designations
NMN adenylyltransferase|OTTHUMP00000001731|pyridine nucleotide adenylyltransferase 1
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