GORASP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GORASP1 transfected lysate.
Western Blot
Lane 1: GORASP1 transfected lysate ( 46.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GORASP1 full-length
Host
Human
Theoretical MW (kDa)
46.5
Interspecies Antigen Sequence
Mouse (82); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GORASP1 antibody (H00064689-B01P) by Western Blots.
SDS-PAGE Gel
GORASP1 transfected lysate.
Western Blot
Lane 1: GORASP1 transfected lysate ( 46.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GORASP1
Entrez GeneID
64689GeneBank Accession#
NM_031899Protein Accession#
NP_114105.1Gene Name
GORASP1
Gene Alias
FLJ23443, GOLPH5, GRASP65, MGC118894, MGC118897, P65
Gene Description
golgi reassembly stacking protein 1, 65kDa
Omim ID
606867Gene Ontology
HyperlinkGene Summary
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq
Other Designations
Golgi peripheral membrane protein p65|Golgi phosphoprotein 5|Golgi reassembly and stacking protein 1|Golgi reassembly and stacking protein, 65 kDa|Golgi reassembly stacking protein 1|OTTHUMP00000161966
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