MRPS25 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPS25 transfected lysate.
Western Blot
Lane 1: MRPS25 transfected lysate ( 20.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPS25 full-length
Host
Human
Theoretical MW (kDa)
20.1
Interspecies Antigen Sequence
Mouse (90); Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPS25 antibody (H00064432-B01P) by Western Blots.
SDS-PAGE Gel
MRPS25 transfected lysate.
Western Blot
Lane 1: MRPS25 transfected lysate ( 20.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPS25
Entrez GeneID
64432GeneBank Accession#
NM_022497Protein Accession#
NP_071942.1Gene Name
MRPS25
Gene Alias
DKFZp313H0817, FLJ00023, MRP-S25, RPMS25
Gene Description
mitochondrial ribosomal protein S25
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq
Other Designations
mitochondrial 28S ribosomal protein S25
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