MRPS25 monoclonal antibody (M01), clone 3E6
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More Files
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Western Blot (Cell lysate)
MRPS25 monoclonal antibody (M01), clone 3E6. Western Blot analysis of MRPS25 expression in PC-12(Cat # L012V1 ).
Western Blot (Cell lysate)
MRPS25 monoclonal antibody (M01), clone 3E6. Western Blot analysis of MRPS25 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot detection against Immunogen (44.77 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MRPS25.
Immunogen
MRPS25 (AAH03590, 1 a.a. ~ 173 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPMKGRFPIRRTLQYLSQGNVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNMTPSPFLRFYLDSGEQVLVDVETKSNKEIMEHIRKILGKNEETLREEEEEKKQLSHPANFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (90); Rat (88)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (44.77 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MRPS25 monoclonal antibody (M01), clone 3E6. Western Blot analysis of MRPS25 expression in PC-12(Cat # L012V1 ).Western Blot (Cell lysate)
MRPS25 monoclonal antibody (M01), clone 3E6. Western Blot analysis of MRPS25 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Recombinant protein)
ELISA
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Gene Info — MRPS25
Entrez GeneID
64432GeneBank Accession#
BC003590Protein Accession#
AAH03590Gene Name
MRPS25
Gene Alias
DKFZp313H0817, FLJ00023, MRP-S25, RPMS25
Gene Description
mitochondrial ribosomal protein S25
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq
Other Designations
mitochondrial 28S ribosomal protein S25
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