INF2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Product Description
Transfected Cell Line
293T
Plasmid
pCMV-C14orf151 full-length
Host
Human
Theoretical MW (kDa)
26
Interspecies Antigen Sequence
Mouse (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-C14orf151 antibody (H00064423-B02) by Western Blots.
SDS-PAGE Gel
INF2 transfected lysate.
Western Blot
Lane 1: INF2 transfected lysate ( 26.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — INF2
Entrez GeneID
64423GeneBank Accession#
NM_032714Protein Accession#
NP_116103Gene Name
INF2
Gene Alias
C14orf151, C14orf173, DKFZp762A0214, FLJ22056, MGC13251, pp9484
Gene Description
inverted formin, FH2 and WH2 domain containing
Omim ID
610982Gene Ontology
HyperlinkGene Summary
Actin filaments grow only when actin monomers have access to the fast-growing barbed end of the filament. The geometry of the filament network depends on the actions of the ARP2/3 complex (MIM 604221) and members of the formin family, such as INF2. The ARP2/3 complex binds to the sides of preexisting filaments and nucleates filaments whose barbed ends are quickly blocked by capping proteins, producing brush-like structures, such as those found at the leading edges of crawling cells. In contrast, formins bind to the barbed ends of growing filaments and protect them from capping, creating long filaments that can be cross-linked into bundles, such as those found in actin cables of yeast. Interaction of formins with actin barbed ends occurs through the formin homology-2 (FH2) domain. FH2 domains accelerate filament nucleation, move with the barbed end as the filament grows, and block capping of the barbed end by proteins such as gelsolin (GSN; MIM 137350). The FH1 domain of formins binds to profilin (see MIM 176610) and accelerates elongation from the FH2-bound barbed ends (Bindschadler and McGrath, 2004 [PubMed 15466701]; Chhabra and Higgs, 2006 [PubMed 16818491]).[supplied by OMIM
Other Designations
inverted formin 2
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