ATG3 monoclonal antibody (M06), clone 1F7
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ATG3.
Immunogen
ATG3 (NP_071933, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (96)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ATG3 monoclonal antibody (M06), clone 1F7 Western Blot analysis of ATG3 expression in Jurkat ( Cat # L017V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ATG3 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — ATG3
Entrez GeneID
64422GeneBank Accession#
NM_022488Protein Accession#
NP_071933Gene Name
ATG3
Gene Alias
APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96
Gene Description
ATG3 autophagy related 3 homolog (S. cerevisiae)
Omim ID
609606Gene Ontology
HyperlinkGene Summary
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM
Other Designations
2610016C12Rik|APG3 autophagy 3-like|Apg3p|autophagy Apg3p/Aut1p-like
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Interactome
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Pathway
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Disease
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