RGS18 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RGS18 transfected lysate.
Western Blot
Lane 1: RGS18 transfected lysate ( 27.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RGS18 full-length
Host
Human
Theoretical MW (kDa)
27.6
Interspecies Antigen Sequence
Mouse (83); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RGS18 antibody (H00064407-B02) by Western Blots.
SDS-PAGE Gel
RGS18 transfected lysate.
Western Blot
Lane 1: RGS18 transfected lysate ( 27.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RGS18
Entrez GeneID
64407GeneBank Accession#
NM_130782.2Protein Accession#
-Gene Name
RGS18
Gene Alias
RGS13
Gene Description
regulator of G-protein signaling 18
Omim ID
607192Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the regulator of G-protein signaling family. This protein is contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
OTTHUMP00000033590|regulator of G-protein signalling 13|regulator of G-protein signalling 18
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