RFWD2 monoclonal antibody (M01), clone 1E4
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RFWD2.
Immunogen
RFWD2 (NP_071902, 632 a.a. ~ 731 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
CLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (97); Rat (99)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RFWD2 monoclonal antibody (M01), clone 1E4. Western Blot analysis of RFWD2 expression in Jurkat.Western Blot (Cell lysate)
RFWD2 monoclonal antibody (M01), clone 1E4 Western Blot analysis of RFWD2 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
RFWD2 monoclonal antibody (M01), clone 1E4. Western Blot analysis of RFWD2 expression in K-562.Western Blot (Cell lysate)
RFWD2 monoclonal antibody (M01), clone 1E4. Western Blot analysis of RFWD2 expression in A-431.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RFWD2 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RFWD2 on NIH/3T3 cell. [antibody concentration 10 ug/ml] -
Gene Info — RFWD2
Entrez GeneID
64326GeneBank Accession#
NM_022457Protein Accession#
NP_071902Gene Name
RFWD2
Gene Alias
COP1, FLJ10416, RNF200
Gene Description
ring finger and WD repeat domain 2
Omim ID
608067Gene Ontology
HyperlinkOther Designations
OTTHUMP00000032911|OTTHUMP00000060611|constitutive photomorphogenic protein (COP1)|putative ubiquitin ligase COP1
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Interactome
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Pathway
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Disease
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