RNF25 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNF25 full-length
Host
Human
Theoretical MW (kDa)
50.6
Interspecies Antigen Sequence
Mouse (82); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNF25 antibody (H00064320-B01) by Western Blots.
SDS-PAGE Gel
RNF25 transfected lysate.
Western Blot
Lane 1: RNF25 transfected lysate ( 50.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNF25
Entrez GeneID
64320GeneBank Accession#
NM_022453.2Protein Accession#
NP_071898.2Gene Name
RNF25
Gene Alias
AO7, FLJ13906
Gene Description
ring finger protein 25
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq
Other Designations
ring finger protein AO7
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