RNF25 monoclonal antibody (M07), clone 1D10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RNF25.
Immunogen
RNF25 (NP_071898.2, 352 a.a. ~ 459 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (84)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.62 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RNF25 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNF25 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — RNF25
Entrez GeneID
64320GeneBank Accession#
NM_022453Protein Accession#
NP_071898.2Gene Name
RNF25
Gene Alias
AO7, FLJ13906
Gene Description
ring finger protein 25
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq
Other Designations
ring finger protein AO7
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