RNF25 MaxPab rabbit polyclonal antibody (D01)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of RNF25 expression in transfected 293T cell line (H00064320-T01) by RNF25 MaxPab polyclonal antibody.
Lane 1: RNF25 transfected lysate(51.20 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of RNF25 transfected lysate using anti-RNF25 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with RNF25 MaxPab mouse polyclonal antibody (B01) (H00064320-B01).
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to RNF25 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human RNF25 protein.
Immunogen
RNF25 (NP_071898.2, 1 a.a. ~ 459 a.a) full-length human protein.
Sequence
MAASASAAAGEEDWVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVCFTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFISLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQHICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS
Host
Rabbit
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (84)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RNF25 expression in transfected 293T cell line (H00064320-T01) by RNF25 MaxPab polyclonal antibody.
Lane 1: RNF25 transfected lysate(51.20 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of RNF25 transfected lysate using anti-RNF25 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with RNF25 MaxPab mouse polyclonal antibody (B01) (H00064320-B01).Immunofluorescence
Immunofluorescence of purified MaxPab antibody to RNF25 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — RNF25
Entrez GeneID
64320GeneBank Accession#
NM_022453.2Protein Accession#
NP_071898.2Gene Name
RNF25
Gene Alias
AO7, FLJ13906
Gene Description
ring finger protein 25
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq
Other Designations
ring finger protein AO7
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Interactome
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