RAB17 monoclonal antibody (M01), clone 2A10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant RAB17.
Immunogen
RAB17 (AAH50426, 1 a.a. ~ 212 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAQAHRTPQPRAAPSQPRVFKLVLLGSGSVGKSSLALRYVKNDFKSILPTVGCAFFTKVVDVGATSLKLEIWDTAGQEKYHSVCHLYFRGANAALLVYDITRKDSFLKAQQWLKDLEEELHPGEVLVMLVGNKTDLSQEREVTFQEGKEFADSQKLLFMETSAKLNHQVSEVFNTVAQELLQRSDEEGQALRGDAAVALNKGPARQAKCCAH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (75); Rat (74)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (49.06 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RAB17 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RAB17 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — RAB17
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Interactome
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Disease
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Publication Reference
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Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Sergi Marco, Matthew Neilson, Madeleine Moore, Arantxa Perez-Garcia, Holly Hall, Louise Mitchell, Sergio Lilla, Giovani R Blanco, Ann Hedley, Sara Zanivan, Jim C Norman.
Nature Communications 2021 Nov; 12(1):6829.
Application:WB-Tr, Human, NCI-H1299 cells.
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Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
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