MLST8 (Human) Recombinant Protein (P01)
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More Files
- More Functions
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Specification
Product Description
Human MLST8 full-length ORF ( NP_071767.3, 1 a.a. - 326 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDPDASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVETGEIKREYGGHQKAVVCLAFNDSVLG
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
62.3
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — MLST8
Entrez GeneID
64223GeneBank Accession#
NM_022372.3Protein Accession#
NP_071767.3Gene Name
MLST8
Gene Alias
GBL, LST8, POP3, WAT1, GbetaL
Gene Description
MTOR associated protein, LST8 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
OTTHUMP00000159046
Other Designations
OTTHUMP00000159046
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Interactome
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Pathway
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Publication Reference
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The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, Khavari PA.
Molecular Cell 2019 Feb; 73(4):830.
Application:Func, Recombinant protein.
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Specific blockade of Rictor-mTOR association inhibits mTORC2 activity and is cytotoxic in glioblastoma.
Benavides-Serrato A, Lee J, Holmes B, Landon KA, Bashir T, Jung ME, Lichtenstein A, Gera J.
PLoS One 2017 Apr; 12(4):e0176599.
Application:Func, inhibitor.
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The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
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