CARD9 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CARD9 transfected lysate.
Western Blot
Lane 1: CARD9 transfected lysate ( 54.23 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CARD9 full-length
Host
Human
Theoretical MW (kDa)
54.23
Interspecies Antigen Sequence
Mouse (85); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CARD9 antibody (H00064170-B01) by Western Blots.
SDS-PAGE Gel
CARD9 transfected lysate.
Western Blot
Lane 1: CARD9 transfected lysate ( 54.23 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CARD9
Entrez GeneID
64170GeneBank Accession#
BC008877Protein Accession#
AAH08877Gene Name
CARD9
Gene Alias
hCARD9
Gene Description
caspase recruitment domain family, member 9
Omim ID
607212Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq
Other Designations
OTTHUMP00000022571|caspase recruitment domain protein 9
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Interactome
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Disease
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