LRAP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ERAP2 transfected lysate.
Western Blot
Lane 1: ERAP2 transfected lysate ( 40.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LRAP full-length
Host
Human
Theoretical MW (kDa)
40.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LRAP antibody (H00064167-B01) by Western Blots.
SDS-PAGE Gel
ERAP2 transfected lysate.
Western Blot
Lane 1: ERAP2 transfected lysate ( 40.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ERAP2
Entrez GeneID
64167GeneBank Accession#
BC017927Protein Accession#
AAH17927Gene Name
ERAP2
Gene Alias
FLJ23633, FLJ23701, FLJ23807, L-RAP, LRAP
Gene Description
endoplasmic reticulum aminopeptidase 2
Omim ID
609497Gene Ontology
HyperlinkGene Summary
Aminopeptidases hydrolyze N-terminal amino acids of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 (MIM 606832) and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2; MIM 190470) (Tanioka et al., 2003 [PubMed 12799365]).[supplied by OMIM
Other Designations
leukocyte-derived arginine aminopeptidase
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Interactome
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Disease
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