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PDF 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00064146-T02
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PDF full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 27
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PDF antibody (H00064146-B01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00064146-T02
    PDF transfected lysate.
    Western Blot
    QC Testing of H00064146-T02
    Lane 1: PDF transfected lysate ( 27.00 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • PDF
  • Gene Alias:
  • -
  • Gene Description:
  • peptide deformylase (mitochondrial)
  • Gene Summary:
  • Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq
  • Other Designations:
  • peptide deformylase,peptide deformylase-like protein
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