CENPK monoclonal antibody (M01), clone 1G2-1B10
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CENPK.
Immunogen
CENPK (AAH05400.1, 1 a.a. ~ 269 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLLIMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKEDLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTLGEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWPPYVELLLRNGIALRHPEDPTRIRLEAFHQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (75); Rat (66)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.33 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CENPK monoclonal antibody (M01), clone 1G2-1B10 Western Blot analysis of CENPK expression in HepG2 ( Cat # L019V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FKSG14 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CENPK on HepG2 cell. [antibody concentration 10 ug/ml] -
Gene Info — CENPK
Entrez GeneID
64105GeneBank Accession#
BC005400Protein Accession#
AAH05400.1Gene Name
CENPK
Gene Alias
AF5alpha, CENP-K, FKSG14, P33, Solt
Gene Description
centromere protein K
Omim ID
611502Gene Ontology
HyperlinkGene Summary
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM
Other Designations
OTTHUMP00000161888|SoxLZ/Sox6 leucine zipper binding protein|SoxLZ/Sox6-binding protein Solt|leucine zipper protein FKSG14
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