SMOC1 monoclonal antibody (M03), clone 8F10
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SMOC1.
Immunogen
SMOC1 (NP_071420, 150 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SVQNKTPVCSGSVTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNIRNS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (95)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SMOC1 monoclonal antibody (M03), clone 8F10 Western Blot analysis of SMOC1 expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SMOC1 expression in transfected 293T cell line by SMOC1 monoclonal antibody (M03), clone 8F10.
Lane 1: SMOC1 transfected lysate(48.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SMOC1 is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SMOC1 on A-431 cell. [antibody concentration 10 ug/ml] -
Gene Info — SMOC1
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Interactome
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Publication Reference
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BMP Signaling Gradient Scaling in the Zebrafish Pectoral Fin.
Mateus R, Holtzer L, Seum C, Hadjivasiliou Z, Dubois M, Jülicher F, Gonzalez-Gaitan M.
Cell Reports 2020 Mar; 30(12):4292.
Application:IF, WB, Zebrafish, Pectoral fin.
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BMP Signaling Gradient Scaling in the Zebrafish Pectoral Fin.
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