CHTF18 monoclonal antibody (M01), clone 1F5
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More Files
- More Functions
Western Blot (Cell lysate)
CHTF18 monoclonal antibody (M01), clone 1F5 Western Blot analysis of CHTF18 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of CHTF18 expression in transfected 293T cell line by CHTF18 monoclonal antibody (M01), clone 1F5.
Lane 1: CHTF18 transfected lysate(107 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHTF18 is approximately 0.1ng/ml as a capture antibody.
Western Blot detection against Immunogen (35.53 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CHTF18.
Immunogen
CHTF18 (AAH18184, 886 a.a. ~ 975 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GVHRPAPRNHEQRLEHIMRRAAREEQPEKDFFGRVVVRSTAVPSAGDTAPEQDSVERRMGTAVGRSEVWFRFNEGVSNAVRRSLYIRDLL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (75); Rat (77)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CHTF18 monoclonal antibody (M01), clone 1F5 Western Blot analysis of CHTF18 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CHTF18 expression in transfected 293T cell line by CHTF18 monoclonal antibody (M01), clone 1F5.
Lane 1: CHTF18 transfected lysate(107 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHTF18 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — CHTF18
Entrez GeneID
63922GeneBank Accession#
BC018184Protein Accession#
AAH18184Gene Name
CHTF18
Gene Alias
C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, Ctf18, RUVBL
Gene Description
CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
C321D2.3 (novel protein)|C321D2.4 (novel protein)|CTF18, chromosome transmission fidelity factor 18 homolog|homolog of yeast CHL12|some homology with holliday junction DNA helicase RUVB like
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Interactome
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Publication Reference
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Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation.
Shiomi Y, Hayashi A, Ishii T, Shinmyozu K, Nakayama J, Sugasawa K, Nishitani H.
Molecular and Cellular Biology 2012 Jun; 32(12):2279.
Application：WB-Tr, Human, HeLa cells.
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Stable interaction between the human PCNA loader complex Ctf18-RFC and DNA polymerase {epsilon} is mediated by the cohesion specific subunits, Ctf18, Dcc1 and Ctf8.
Murakami T, Takano R, Takeo S, Taniguchi R, Ogawa K, Ohashi E, Tsurimoto T.
The Journal of Biological Chemistry 2010 Nov; 285(45):34608.
Application：WB-Ce, Human, HeLa cells.
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Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation.
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