MRPS35 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPS35 transfected lysate
Western Blot
Lane 1: MRPS35 transfected lysate ( 25.08 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPS35 full-length
Host
Human
Theoretical MW (kDa)
25.08
Interspecies Antigen Sequence
Mouse (76); Rat (77)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPS35 antibody (H00060488-B01) by Western Blots.
SDS-PAGE Gel
MRPS35 transfected lysate
Western Blot
Lane 1: MRPS35 transfected lysate ( 25.08 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPS35
Entrez GeneID
60488GeneBank Accession#
BC015862Protein Accession#
AAH15862Gene Name
MRPS35
Gene Alias
DKFZp762P093, HDCMD11P, MDS023, MGC104278, MRP-S28, MRPS28
Gene Description
mitochondrial ribosomal protein S35
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq
Other Designations
mitochondrial ribosomal protein S28
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