AFAP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AFAP1 transfected lysate.
Western Blot
Lane 1: AFAP1 transfected lysate ( 80.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AFAP full-length
Host
Human
Theoretical MW (kDa)
80.7
Interspecies Antigen Sequence
Mouse (91); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AFAP antibody (H00060312-B01) by Western Blots.
SDS-PAGE Gel
AFAP1 transfected lysate.
Western Blot
Lane 1: AFAP1 transfected lysate ( 80.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AFAP1
Entrez GeneID
60312GeneBank Accession#
BC032777.1Protein Accession#
AAH32777.1Gene Name
AFAP1
Gene Alias
AFAP, AFAP-110, FLJ56849
Gene Description
actin filament associated protein 1
Omim ID
608252Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000155170|actin filament-associated protein, 110 kDa
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