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ALOXE3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00059344-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ALOXE3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 78.32
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ALOXE3 antibody (H00059344-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00059344-T01
    ALOXE3 transfected lysate.
    Western Blot
    QC Testing of H00059344-T01
    Lane 1: ALOXE3 transfected lysate ( 78.32 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • ALOXE3
  • Gene Alias:
  • E-LOX,MGC119694,MGC119695,MGC119696,eLOX3
  • Gene Description:
  • arachidonate lipoxygenase 3
  • Gene Summary:
  • This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene
  • Other Designations:
  • epidermal lipoxygenase,lipoxygenase-3
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