SEMA4G 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SEMA4G transfected lysate.
Western Blot
Lane 1: SEMA4G transfected lysate ( 13.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SEMA4G full-length
Host
Human
Theoretical MW (kDa)
14.08
Interspecies Antigen Sequence
Mouse (33); Rat (33)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SEMA4G antibody (H00057715-B02) by Western Blots.
SDS-PAGE Gel
SEMA4G transfected lysate.
Western Blot
Lane 1: SEMA4G transfected lysate ( 13.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SEMA4G
Entrez GeneID
57715GeneBank Accession#
BC020960Protein Accession#
AAH20960Gene Name
SEMA4G
Gene Alias
FLJ20590, KIAA1619, MGC102867
Gene Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G
Gene Ontology
HyperlinkGene Summary
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene and the gene for mitochondrial ribosomal protein L43 overlap at map location 10q24.31 and are transcribed in opposite directions. [provided by RefSeq
Other Designations
OTTHUMP00000020312|semaphorin 4G
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Interactome
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Pathway
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Disease
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