USP28 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
USP28 transfected lysate.
Western Blot
Lane 1: USP28 transfected lysate ( 64.24 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-USP28 full-length
Host
Human
Theoretical MW (kDa)
64.24
Interspecies Antigen Sequence
Mouse (88); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-USP28 antibody (H00057646-B01) by Western Blots.
SDS-PAGE Gel
USP28 transfected lysate.
Western Blot
Lane 1: USP28 transfected lysate ( 64.24 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — USP28
Entrez GeneID
57646GeneBank Accession#
BC065928.1Protein Accession#
AAH65928.1Gene Name
USP28
Gene Alias
KIAA1515
Gene Description
ubiquitin specific peptidase 28
Omim ID
610748Gene Ontology
HyperlinkGene Summary
The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).[supplied by OMIM
Other Designations
ubiquitin specific protease 28
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