SRGAP1 monoclonal antibody (M03), clone 5D2
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SRGAP1.
Immunogen
SRGAP1 (NP_065813, 952 a.a. ~ 1050 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SRGAP1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SRGAP1 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — SRGAP1
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Interactome
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Pathway
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Publication Reference
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FBP17 and CIP4 recruit SHIP2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis.
Chan Wah Hak L, Khan S, Di Meglio I, Law AL, Häsler SL, Quintaneiro LM, Ferreira APA, Krause M, McMahon HT, Boucrot E.
Nature Cell Biology 2018 Sep; 20(9):1023.
Application:IS, Monkey, BSC1 cells.
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A link between the nuclear-localized srGAP3 and the SWI/SNF chromatin remodeler Brg1.
Dai YK, Ma Y, Chen K, Mi YJ, Fu HL, Cui DX, Jin WL.
Molecular and Cellular Neurosciences 2014 Feb; 60:10.
Application:WB, Rat, Cortical neurons.
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FBP17 and CIP4 recruit SHIP2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis.
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