RPTOR 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RPTOR full-length
Host
Human
Theoretical MW (kDa)
41.8
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RPTOR antibody (H00057521-B01) by Western Blots.
SDS-PAGE Gel
RPTOR transfected lysate.
Western Blot
Lane 1: RPTOR transfected lysate ( 43.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RPTOR
Entrez GeneID
57521GeneBank Accession#
BC064515Protein Accession#
AAH64515Gene Name
RPTOR
Gene Alias
KOG1, Mip1
Gene Description
regulatory associated protein of MTOR, complex 1
Omim ID
607130Gene Ontology
HyperlinkGene Summary
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|regulatory associated protein of mTOR
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Interactome
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Disease
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