RPTOR (Human) IP-WB Antibody Pair
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More Files
- More Functions
Immunoprecipitation of RPTOR transfected lysate using rabbit polyclonal anti-RPTOR and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RPTOR.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of RPTOR transfected lysate using rabbit polyclonal anti-RPTOR and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RPTOR.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-RPTOR (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-RPTOR (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — RPTOR
Entrez GeneID
57521Gene Name
RPTOR
Gene Alias
KOG1, Mip1
Gene Description
regulatory associated protein of MTOR, complex 1
Omim ID
607130Gene Ontology
HyperlinkGene Summary
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|regulatory associated protein of mTOR
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Interactome
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Pathway
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Disease
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