RNF150 monoclonal antibody (M01), clone 5D11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RNF150.
Immunogen
RNF150 (NP_065775, 67 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LHTEKTECGRYGEHSPKQDARGEVVMASSAHDRLACDPNTKFAAPTRGKNWIALIPKGNCTYRDKIRNAFLQNASAVVIFNVGSNTNETITMPHAGVEDIVAIMIPEPKG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (95)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RNF150 expression in transfected 293T cell line by RNF150 monoclonal antibody (M01), clone 5D11.
Lane 1: RNF150 transfected lysate(34.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNF150 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — RNF150
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Interactome
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Disease
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