SMURF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SMURF1 transfected lysate.
Western Blot
Lane 1: SMURF1 transfected lysate ( 83.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SMURF1 full-length
Host
Human
Theoretical MW (kDa)
83.4
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SMURF1 antibody (H00057154-D01P) by Western Blots.
SDS-PAGE Gel
SMURF1 transfected lysate.
Western Blot
Lane 1: SMURF1 transfected lysate ( 83.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SMURF1
Entrez GeneID
57154GeneBank Accession#
NM_181349Protein Accession#
NP_851994.1Gene Name
SMURF1
Gene Alias
KIAA1625
Gene Description
SMAD specific E3 ubiquitin protein ligase 1
Omim ID
605568Gene Ontology
HyperlinkGene Summary
This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq
Other Designations
E3 ubiquitin ligase SMURF1|Smad ubiquitination regulatory factor 1|Smad-specific E3 ubiquitin ligase 1
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Interactome
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Pathway
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