DAZ4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DAZ4 transfected lysate.
Western Blot
Lane 1: DAZ4 transfected lysate ( 44.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DAZ4 full-length
Host
Human
Theoretical MW (kDa)
44.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DAZ4 antibody (H00057135-B01) by Western Blots.
SDS-PAGE Gel
DAZ4 transfected lysate.
Western Blot
Lane 1: DAZ4 transfected lysate ( 44.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DAZ4
Entrez GeneID
57135GeneBank Accession#
NM_020420Protein Accession#
NP_065153Gene Name
DAZ4
Gene Alias
DAZ, DAZ1, pDP1680, pDP1681
Gene Description
deleted in azoospermia 4
Gene Ontology
HyperlinkGene Summary
This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
deleted in azoospermia 1
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Disease
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