PLXDC1 monoclonal antibody (M01), clone 4B10
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PLXDC1.
Immunogen
PLXDC1 (NP_065138, 313 a.a. ~ 421 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
CMSSDLTFNCSWCHVLQRCSSGFDRYRQEWMDYGCAQEAEGRMCEDFQDEDHDSASPDTSFSPYDGDLTTTSSSLFIDSLTTEDDTKLNPYAGGDGLQNNLSPKTKGTP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (82)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PLXDC1 expression in transfected 293T cell line by PLXDC1 monoclonal antibody (M01), clone 4B10.
Lane 1: PLXDC1 transfected lysate(55.778 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PLXDC1 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PLXDC1 over-expressed 293 cell line, cotransfected with PLXDC1 Validated Chimera RNAi ( Cat # H00057125-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PLXDC1 monoclonal antibody (M01), clone 4B10 (Cat # H00057125-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — PLXDC1
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Disease
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