C12orf5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
C12orf5 transfected lysate.
Western Blot
Lane 1: C12orf5 transfected lysate ( 29.81 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-C12orf5 full-length
Host
Human
Theoretical MW (kDa)
29.81
Interspecies Antigen Sequence
Mouse (72)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-C12orf5 antibody (H00057103-B01) by Western Blots.
SDS-PAGE Gel
C12orf5 transfected lysate.
Western Blot
Lane 1: C12orf5 transfected lysate ( 29.81 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — C12orf5
Entrez GeneID
57103GeneBank Accession#
NM_020375.2Protein Accession#
NP_065108.1Gene Name
C12orf5
Gene Alias
TIGAR
Gene Description
chromosome 12 open reading frame 5
Omim ID
610775Gene Ontology
HyperlinkGene Summary
This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq
Other Designations
TP53-induced glycolysis and apoptosis regulator|transactivated by NS3TP2 protein
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