AGTRAP monoclonal antibody (M02), clone 1G2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant AGTRAP.
Immunogen
AGTRAP (NP_065083, 108 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
HMYRERGGELLVHTGFLGSSQDRSAYQTIDSAEAPADPFAVPEGRSQDARGS
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (31.46 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
AGTRAP monoclonal antibody (M02), clone 1G2 Western Blot analysis of AGTRAP expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of AGTRAP expression in transfected 293T cell line by AGTRAP monoclonal antibody (M02), clone 1G2.
Lane 1: AGTRAP transfected lysate(16.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to AGTRAP on formalin-fixed paraffin-embedded human prostate. [antibody concentration 0.8 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AGTRAP is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of AGTRAP over-expressed 293 cell line, cotransfected with AGTRAP Validated Chimera RNAi ( Cat # H00057085-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AGTRAP monoclonal antibody (M02), clone 1G2 (Cat # H00057085-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — AGTRAP
Entrez GeneID
57085GeneBank Accession#
NM_020350Protein Accession#
NP_065083Gene Name
AGTRAP
Gene Alias
ATRAP, MGC29646
Gene Description
angiotensin II receptor-associated protein
Omim ID
608729Gene Ontology
HyperlinkGene Summary
This gene encodes a transmembrane protein localized to the plasma membrane and perinuclear vesicular structures. The gene product interacts with the angiotensin II type I receptor and negatively regulates angiotensin II signaling. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
ATI receptor-associated protein|OTTHUMP00000002269|OTTHUMP00000002270|OTTHUMP00000002271|angiotensin II, type I receptor-associated protein
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Interactome
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Disease
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