SPIRE1 monoclonal antibody (M01), clone 4C5
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant SPIRE1.
Immunogen
SPIRE1 (NP_064533, 482 a.a. ~ 583 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (93)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.96 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SPIRE1 expression in transfected 293T cell line by SPIRE1 monoclonal antibody (M01), clone 4C5.
Lane 1: SPIRE1 transfected lysate(15 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPIRE1 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — SPIRE1
Entrez GeneID
56907GeneBank Accession#
NM_020148Protein Accession#
NP_064533Gene Name
SPIRE1
Gene Alias
MGC150621, MGC150622, Spir-1
Gene Description
spire homolog 1 (Drosophila)
Omim ID
609216Gene Ontology
HyperlinkGene Summary
Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM
Other Designations
spire homolog 1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Annexin A2-Dependent Polymerization of Actin Mediates Endosome Biogenesis.
Morel E, Parton RG, Gruenberg J.
Developmental Cell 2009 Mar; 16(3):445.
Application:WB, Hamster, BHK cells.
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Actin S-nitrosylation inhibits neutrophil beta2 integrin function.
Thom SR, Bhopale VM, Mancini DJ, Milovanova TN.
The Journal of Biological Chemistry 2008 Feb; 283(16):10822.
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Annexin A2-Dependent Polymerization of Actin Mediates Endosome Biogenesis.
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