MDM1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MDM1 transfected lysate.
Western Blot
Lane 1: MDM1 transfected lysate ( 80.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MDM1 full-length
Host
Human
Theoretical MW (kDa)
80.7
Interspecies Antigen Sequence
Mouse (69); Rat (68)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MDM1 antibody (H00056890-B01) by Western Blots.
SDS-PAGE Gel
MDM1 transfected lysate.
Western Blot
Lane 1: MDM1 transfected lysate ( 80.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MDM1
Entrez GeneID
56890GeneBank Accession#
NM_017440Protein Accession#
NP_059136Gene Name
MDM1
Gene Alias
FLJ95264
Gene Description
Mdm1 nuclear protein homolog (mouse)
Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. In mouse several transcripts have been described for this gene which result from alternative polyadenylation, splicing and exon usage. [provided by RefSeq
Other Designations
Mdm4, transformed 3T3 cell double minute 1, p53 binding protein|mouse Mdm1 nuclear protein homolog|nuclear protein MDM1|nuclear protein double minute 1
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