MDM1 MaxPab mouse polyclonal antibody (B01)
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of MDM1 expression in transfected 293T cell line (H00056890-T01) by MDM1 MaxPab polyclonal antibody.
Lane 1: MDM1 transfected lysate(78.54 KDa).
Lane 2: Non-transfected lysate.
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human MDM1 protein.
Immunogen
MDM1 (NP_059136, 1 a.a. ~ 714 a.a) full-length human protein.
Sequence
MPVRFKGLSEYQRNFLWKKSYLSESCNSSVGRKYPWAGLRSDQLGITKEPSFISKRRVPYHDPQISKSLEWNGAISESNVVASPEPEAPETPKSQEAEQKDVIQERVHSLEASRVPKRTRSHSADSRAEGASDVENNEGVTNHTPVNENVELEHSTKVLSENVDNGLDRLLRKKAGLTVVPSYNALRNSEYQRQFVWKTSKETAPAFAANQVFHNKSQFVPPFKGNSVIHETEYKRNFKGLSPVKEPKLRNDLRENRNLETVSPERKSNKIDDRLKLEAEMELKDLHQPKRKLTPWKHQRLGKVNSEYRAKFLSPAQYLYKAGAWTHVKGNMPNQVKELREKAEFYRKRVQGTHFSRDHLNQILSDSNCCWDVSSTTSSEGTVSSNIRALDLAGDPTSHKTLQKCPSTEPEEKGNIVEEQPQKNTTEKLGVSAPTIPVRRRLAWDTENTSEDVQKQPGEKEEEDDNEEEGDRKTGKQAFMGEQEKLDVHEKSKADKMKEGSDSSVSSEKGGRLPTPKLRELGGIQRTHHDLTTPAVGGAVLVSPSKMKPPALEQRKRMTSQDCLETSKNDFTKKESRAVSLLTSPAAGIKTVDPLPLREDSEDNIHKFAEATLPVSKIPKYPTNPPGQLPSPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTLARAKKRKENFWGKT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (69); Rat (68)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MDM1 expression in transfected 293T cell line (H00056890-T01) by MDM1 MaxPab polyclonal antibody.
Lane 1: MDM1 transfected lysate(78.54 KDa).
Lane 2: Non-transfected lysate.
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Gene Info — MDM1
Entrez GeneID
56890GeneBank Accession#
NM_017440Protein Accession#
NP_059136Gene Name
MDM1
Gene Alias
FLJ95264
Gene Description
Mdm1 nuclear protein homolog (mouse)
Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. In mouse several transcripts have been described for this gene which result from alternative polyadenylation, splicing and exon usage. [provided by RefSeq
Other Designations
Mdm4, transformed 3T3 cell double minute 1, p53 binding protein|mouse Mdm1 nuclear protein homolog|nuclear protein MDM1|nuclear protein double minute 1
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Interactome
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Publication Reference
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MDM1 is a microtubule-binding protein that negatively regulates centriole duplication.
Van de Mark D, Kong D, Loncarek J, Stearns T.
Molecular Biology of the Cell 2015 Nov; 26(21):3788.
Application：IF, WB-Ce, Human, RPE-1 cells.
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MDM1 is a microtubule-binding protein that negatively regulates centriole duplication.
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