RAD18 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RAD18 transfected lysate.
Western Blot
Lane 1: RAD18 transfected lysate ( 56.20 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RAD18 full-length
Host
Human
Theoretical MW (kDa)
56.2
Interspecies Antigen Sequence
Mouse (65); Rat (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RAD18 antibody (H00056852-D01P) by Western Blots.
SDS-PAGE Gel
RAD18 transfected lysate.
Western Blot
Lane 1: RAD18 transfected lysate ( 56.20 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RAD18
Entrez GeneID
56852GeneBank Accession#
NM_020165.2Protein Accession#
NP_064550.2Gene Name
RAD18
Gene Alias
RNF73
Gene Description
RAD18 homolog (S. cerevisiae)
Omim ID
605256Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq
Other Designations
RAD18, S. cerevisiae, homolog|postreplication repair protein hRAD18p
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