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RAD18 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00056852-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RAD18 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 54.56
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RAD18 antibody (H00056852-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00056852-T01
    RAD18 transfected lysate.
    Western Blot
    QC Testing of H00056852-T01
    Lane 1: RAD18 transfected lysate ( 54.56 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • RAD18
  • Gene Alias:
  • RNF73
  • Gene Description:
  • RAD18 homolog (S. cerevisiae)
  • Gene Summary:
  • The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq
  • Other Designations:
  • RAD18, S. cerevisiae, homolog,postreplication repair protein hRAD18p
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