This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity:
Human
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of RAD18 transfected lysate using rabbit polyclonal anti-RAD18 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RAD18.
Supplied Product:
Antibody pair set content: 1. Antibody pair for IP: rabbit polyclonal anti-RAD18 (300 ul) 2. Antibody pair for WB: mouse purified polyclonal anti-RAD18 (50 ug)
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq
Other Designations:
RAD18, S. cerevisiae, homolog,postreplication repair protein hRAD18p