INPP5E 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
INPP5E transfected lysate.
Western Blot
Lane 1: INPP5E transfected lysate ( 70.95 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-INPP5E full-length
Host
Human
Theoretical MW (kDa)
70.95
Interspecies Antigen Sequence
Mouse (76); Rat (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-INPP5E antibody (H00056623-B01) by Western Blots.
SDS-PAGE Gel
INPP5E transfected lysate.
Western Blot
Lane 1: INPP5E transfected lysate ( 70.95 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — INPP5E
Entrez GeneID
56623GeneBank Accession#
NM_019892.3Protein Accession#
NP_063945.2Gene Name
INPP5E
Gene Alias
MGC117201, PPI5PIV
Gene Description
inositol polyphosphate-5-phosphatase, 72 kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4, 5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. [provided by RefSeq
Other Designations
OTTHUMP00000022575|inositol polyphosphate-5-phosphatase E|phosphatidylinositol (4,5) bisphosphate 5-phosphatase|phosphatidylinositol polyphosphate 5-phosphatase type IV
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Interactome
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