DIABLO 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DIABLO transfected lysate.
Western Blot
Lane 1: DIABLO transfected lysate ( 26.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DIABLO full-length
Host
Human
Theoretical MW (kDa)
26.4
Interspecies Antigen Sequence
Mouse (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DIABLO antibody (H00056616-B01) by Western Blots.
SDS-PAGE Gel
DIABLO transfected lysate.
Western Blot
Lane 1: DIABLO transfected lysate ( 26.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DIABLO
Entrez GeneID
56616GeneBank Accession#
NM_019887Protein Accession#
NP_063940Gene Name
DIABLO
Gene Alias
DIABLO-S, FLJ10537, FLJ25049, SMAC, SMAC3
Gene Description
diablo homolog (Drosophila)
Omim ID
605219Gene Ontology
HyperlinkGene Summary
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq
Other Designations
0610041G12Rik|diablo|direct IAP-binding protein with low pI|mitochondrial Smac protein|second mitochondria-derived activator of caspase
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Interactome
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Disease
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