PARD3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PARD3 transfected lysate.
Western Blot
Lane 1: PARD3 transfected lysate ( 149.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PARD3 full-length
Host
Human
Theoretical MW (kDa)
149.7
Interspecies Antigen Sequence
Mouse (89); Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PARD3 antibody (H00056288-B01) by Western Blots.
SDS-PAGE Gel
PARD3 transfected lysate.
Western Blot
Lane 1: PARD3 transfected lysate ( 149.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PARD3
Entrez GeneID
56288GeneBank Accession#
BC071566Protein Accession#
AAH71566Gene Name
PARD3
Gene Alias
ASIP, Baz, Bazooka, FLJ21015, PAR3, PAR3alpha, PARD3A, SE2-5L16, SE2-5LT1, SE2-5T2
Gene Description
par-3 partitioning defective 3 homolog (C. elegans)
Omim ID
606745Gene Ontology
HyperlinkGene Summary
PARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 (MIM 116952) mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange factors (GEFs; see MIM 606057) and GTPase-activating proteins (GAPs; see MIM 604980), interacts with a large set of effector proteins that typically contain a CDC42/RAC (MIM 602048)-interactive binding (CRIB) domain.[supplied by OMIM
Other Designations
OTTHUMP00000019428|atypical PKC isotype-specific interacting protein|partitioning-defective protein 3 homolog
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Interactome
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Pathway
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Disease
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