RNF20 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RNF20 transfected lysate.
Western Blot
Lane 1: RNF20 transfected lysate ( 107.36 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNF20 full-length
Host
Human
Theoretical MW (kDa)
107.36
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNF20 antibody (H00056254-B01) by Western Blots.
SDS-PAGE Gel
RNF20 transfected lysate.
Western Blot
Lane 1: RNF20 transfected lysate ( 107.36 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNF20
Entrez GeneID
56254GeneBank Accession#
NM_019592.5Protein Accession#
NP_062538.5Gene Name
RNF20
Gene Alias
BRE1, BRE1A, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668, hBRE1
Gene Description
ring finger protein 20
Omim ID
607699Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3. [provided by RefSeq
Other Designations
BRE1 E3 ubiquitin ligase homolog|homolog of S. cerevisiae BRE1
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